cpa200

CPA200™

Semi-automated puncher for Human DNA samples processing and traceability.
 
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Please contact Copan for product codes and product features availability in your Country.

The intended use of the Card Processing Automation 200™ (CPA200™) is to fill a 96-wells PCR plate with human’s saliva or blood samples extracted from COPAN NUCLEIC-CARD™ or other compatible sample collection devices.

Extraction is performed trough a cylindrical punch head (puncher), which detaches a paper disk from the card containing the DNA sample and places it into one well of the PCR plate underneath.

CPA200™ is a semi-automatic instrument: the user manually loads a card, chooses the position where to extract the DNA sample through a laser pointer and launches the punching operation, which is automatically performed by the system.

CPA200™ is compatible with all the sample collection devices currently present in the market, including COPAN NUCLEIC-CARD™, Whatman FTA™ cards, Bode Buccal DNA Collector™ and Bode Buccal 2™. Performances are optimized when processing Copan framed NUCLEIC-CARD™ with cleaning area: the user has the possibility to load a card on the integrated card holder, which automatically handles the card when punching and performs the cleaning strike to prevent any DNA carry-over effect.

A 96-wells PCR plate can contain up to 96 different Human DNA samples: for this reason, CPA200™ equips barcode readers to ensure the traceability of processed samples and a vision system in order to check the presence of disks inside plate wells.

Until today, CPA200™ instruments have been installed in forensic laboratories for Human DNA database-related activities. Future applications will belong to the clinical field (newborn screening and more…).

 

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  • Brochure

    High Punching Accuracy

    Extraction of samples from COPAN NUCLEIC-CARD™ or other compatible sample collection devices is performed through a cylindrical punch head (puncher), which detaches a paper disk form the card containing the DNA sample and places it into one well of the PCR plate underneath.

    The position where to punch the DNA sample on a card is defined manually by the user through two laser pointers.
    1.2 mm puncher is complimentary with the standard system, while different punch sizes are available separately:

    • 2mm
    • 3mm
    • 4mm


    The instrument is capable to perform up to one puncher cleaning strike, preventing DNA carry-over from a sample to the next one. Performances are optimized when processing COPAN framed NUCLEIC-CARD™ with cleaning area: the user has the possibility to load a card on the integrated card holder, which automatically handles the card when punching and performs the cleaning strike.
    Moreover, the instrument integrates a vacuum pump in order to aspirate card debris from the punching area and minimize any cross-contamination phenomena, and an ionizer to remove statics and avoid sample jumping from well to well.
    In order to improve the easiness of use, a footswitch pedal can be integrated with the instrument.

    Cards and PCR Plates Flexibility

    CPA200™ is compatible with all the sample collection devices currently present in the market, including Copan NUCLEIC-CARD™, Whatman FTA™ cards, Bode Buccal DNA Collector™ and Bode Buccal 2™.
    The system allows the possibility to re-process already punched cards.
    Plate holder can be customized in order to process a specific 96-wells PCR plate type (except for deep-well plates).

    Samples and ID Traceability

    A 96-wells PCR plate can contain up to 96 different Human DNA samples: for this reason, CPA200™ equips barcode readers to ensure the traceability of processed cards and PCR plates.
    A dedicated vision system can automatically check the presence of the punched sample into the relevant well by taking pictures of the well both before and after punching.

    System Integration

    CPA200™ can be connected with a LIMS or with a separate computer to allow both import and export functionalities.
    The instrument can import .txt worklists containing information about PCR plates barcodes, plates layouts, cards barcodes and number of punches per well, and assist the user in performing the run.
    It is possible to export reports of the completed run with the following formats:

    • .pdf format
    • 3130 and 3500 AB Genetic Analyzer sequencer compatible files (.txt format)
    • LIMS compatible files (.txt format)

    Specifications

    Specifications: 

    DIMENSIONS:610mm (L) x 545mm (W) x 675mm (H)
    WEIGHT: 85kg
    INTEGRATED COMPONENTS: Vacuum pump for card debrises aspiration. Footswitch pedal.
    PC (INCLUDED): Model: ASEM HT2000-TF/S1: LCD TFT 10,4" 4:3, Touch-Screen 10,4", Intel® Atom™ dual core D2550 1,86 GHz, 1GB RAM. OS: Linux Ubuntu.
    PERIPHERALS: 10,4'' Touch screen, keyboard with touchpad, USB (2). Possibility to plug keyboard with foreign language layout.
    NETWORK: 1 x Ethernet (1 Gbit/s)
    CONNECTIVITY: The instrument can be connected to a LIMS and allow both import and export of files.
    EXPORTABLE FILES: The instrument can export files in.txt format suitable for the 3130 and 3500 sequencers. It's also possible to export reports as PDF files. The instrument can both export and import LIMS-compatible files.
    USER INTERFACE LANGUAGE: English
    MAIN SUPPLY VOLTAGE: 208 – 240 VAC
    FREQUENCY: 50/60 Hz
    ELECTRICAL POWER: 400 W (peak)

    Operating conditions:

    MAX ALTITUDE: 3000m
    HUMIDITY: 15% to 90%
    INDOOR TEMPERATURE: 5°C to 35°C. 

    For indoor use only 

    Features & compatibility:

    SUPPORTED CARD FORMATS: All card formats supported.Possibility to re-process already punched cards.
    PROCESSABLE SAMPLE TYPES: Saliva samples deposited with all collection devices on both indicating and non-indicating cards. Bode Buccal DNA Collector and Bode Buccal 2.
    AVAILABLE PUNCH SIZES: 1.2mm, 2mm, 3mm, 4mm
    READABLE BARCODE TYPES: 1D e Stacked: IL 2/5, Code 128, Code 39, Code 93 EAN/UPC, PDF417, Micro PDF417, Pharmacode, GS1 DATABAR (RSS) family, and many more. 2D: Data Matrix, QR Code, Maxicode, Aztec, Microglyph.
    PLATE TYPES: All plates types (except deep-weel plates) are integrable with the system by request to the manufacturer.
    CLEANING STRIKE CAPABILITY: Optimized protocol using NUCLEIC-CARD™ FRAMED w/ cleaning area: 1st cleaning punch performed automatically; possibility to use again the same cleaning area using the external laser pointer. 
    ALL OTHER CARDS: cleaning procedure can be performed using a cleaning card or on a blank area of the processing card using the external or the internal laser pointer. Maximum 1 cleaning strike after punching each sample. 

    Capacity:

    PLATES CAPACITY PER SINGLE RUN: 2 plates (192 cards).

    Camera features:

    MODEL: IDS Direct XS
    RESOLUTION: 2592 (h) x 1944 (v)

    Scientific Studies

    MSDS

    MSDS

     

  • High punching accuracy

    The punching system ensures the disk is delivered in the correct well of the PCR plate and minimizes cross-contamination issues. Different sizes of punching tool are available.

    Cards and PCR plates flexibility

    CPA200™ is compatible with a variety of card shapes and dimensions and can be customized for processing different types of 96-wells PCR plates.

    Samples and ID traceability

    ID of the processed samples and plates are recorded through barcode readers. An integrated camera checks the sample presence into the well after punching.

    System integration

    The embedded computer can be integrated into laboratory SOPs and LIMS with customizable, exportable files and reports.

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